One scaffold, several approaches: tackling cancer diseases with Nanofitins @PEGS2022
Mathieu Cinier to present a poster showing latest data of Nanofitin used as cancer targeted therapies. Nanofitins display multiple specifications making them very attractive as alternative scaffolds. More specifically, they show fast and deep tissue penetration, thanks to their small size, and easy conjugation for multispecific assembly. Moreover, it is very easy to conjugate them to other payloads, like radionuclides, cytotoxics …
Development of multispecific assemblies of Nanofitins® with anti-tumor activities @ PEGS Europe 2021
Ms. Perrine Jacquot, Ph.D. student working at Affilogic and at the Western Cancer Institute (Institut de Cancérologie de l’Ouest, Saint-Herblain, France) presented her latest results in November in Barcelona during PEGS Europe 2021. Nanofitins® are small and robust affinity proteins considered as alternatives to antibodies. These proteins can easily be associated with each other in order to increase avidity or multi-specificity. In the development of anti-tumor strategies, the assembly of Nanofitins® with various activities (targeting of receptors / soluble factors, recruitment of immune cells, etc) allows the targeting of multiple pathways involved in cancer progression and aims at the development of an effective anti-tumor response with limited toxicities.
Nanofitin-Antibody fusion as a novel multispecific platform: providing extra specificities while maintaining developability
Webinar Oxford Global, June 2019
Building on antibody expertise, many different bispecific formats have been engineered to allow the additional neutralization of an escaping pathway or the recruitment of effectors, but often at the expense of the overall physico-chemical properties of the biologic itself compared to the actual state of the art for monoclonal antibodies. Nanofitin-Antibody fusion is proposed as a novel multispecific platform, whereby additional targeting specificities are provided by the fusion of Nanofitins to existing antibodies without altering their initial physico-chemical properties.
Nanofitin is a small (7 kDa) single chain, hyperstable, cysteine-free, and highly soluble alternative scaffold that can be custom-engineered for a defined targeting specificity. Nanofitins are easily assembled with each other or with third-party molecules such as antibodies by simple, rapid and proven methods to design an ideal targeted molecule. With the strong rational behind the targeting of multiple disease pathways, the trend is moving smoothly from the design of bispecific to multi-specific antibodies, which could be easily adapted with Nanofitins. Thanks to their small size, Nanofitins can be plugged on an antibody at different locations, either as a single module or as an assembly, while still providing a minimal hindrance on the antibody that retain its functionality. Resulting Nanofitin-Antibody fusions benefit from the high stability and solubility of the Nanofitin scaffold, providing to the Nanofitin-based multi-specific platform a developablility advantage.
Development of multispecific assemblies of Nanofitins with anti-tumor activities @ the 8th Antibody Industrial Symposium 2020
Digital, November 2020
Affilogic is developing a new anti-cancer therapeutic approach. Different multispecific assemblies of Nanofitins are developed with a variation of the modules that compose them. In order to potentiate the anti-tumor response, the choice of Nanofitin modules to assemble focuses on those with activities involved in cancer synergistic signaling pathways. In the development of multispecific anti-tumor proteins, Nanofitins provide several advantages. Indeed, thanks to their good tolerance to engineering modifications as well as the non-involvement of their N- and C-terminal ends in the binding, their multimerizations with other molecules / proteins / toxins / antibodies and Nanofitins are facilitated and used in order to do multi-targeting and/or increase avidity. Moreover the robustness of these proteins limits the possibilities of their deactivation in the presence of an aggressive tumor environment. In addition, the absence of cysteines and post-translational modifications allows the production in prokaryotic system of several Nanofitins grouped in a single molecule, which will bring an attractive cost in the multimers development. Finally, their low molecular weight promotes their diffusion within the tumor mass and gives them a short plasma half-life allowing the elimination in a few minutes of unbound proteins which is interesting for reducing side effects.
This project was partially sponsored from January 2018 to December 2020 by a grant from the European Regional Development Fund (ERDF / FEDER) via the Region Pays de Loire.